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Related post: the production of rickettsial antigens Order Anaprox by their reactivity with polyclonal sera raised against intact R. rickettsii . Thus far, three recombinant plasmids and one recombinant phage, which encode rickettsial antigens, have been identified. The insert from one Purchase Anaprox Online of the recombinant plasmids was subcloned into pUC8 and pUC9. Cells harboring these plasmids have been used as vaccines in mice in efforts to prevent the death of mice caused by intravenous injection with viable R. rickettsii . Cells harboring the pUC8 subclone offered some protection as 50% of the mice vaccinated in this group survived challenge with a 2 LD R. rickettsii. 50 of 12-23 GPC 90^-917 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00442-01 LMSF PERIOD COVERED October 1 , 1984, to September 30, 1985 TITLE OF PROJECT (80 characters or less. Title rrtust lit on one line between the borders.) Immunoblology of Guinea Pig Inclusion Conjunctivitis PRINCIPAL INVESTIGATOR (List other professional personnel below the Principal Investigator ) (Name, title, laboratory, and institute affiliation) PI: N. G. Watkins Staff Fellow LMSF, NIAID Others: H. D. Caldwell F. E. Nano W. J. Hadlow Research Microbiologist Staff Fellow Research Veterinarian LMSF, NIAID LMSF, NIAID LPB, NIAID COOPERATING UNITS (if any) LAB/BRANCH Laboratory of Microbial Structure and Function, Hamilton, MT 59840 SECTION INSTITUTE AND LOCATION NIAID. NIH, Bethesda, MD 20205 TOTAL MAN-YEARS: 1.75 PROFESSIONAL: 1.15 OTHER: 0.6 CHECK APPROPRIATE B0X{ES) D (a) Human subjects D (a1) Minors □ {a2) Interviews D Generic Anaprox (b) Human tissues \3 Purchase Anaprox (c) Neither SUMMARY OF WORK Buy Cheap Anaprox (Use standard unreduced type. Do not exceed the space provided.) The pathogenesis of recurrent ocular chlamydial infections is thought to be immunologically mediated Buy Anaprox Online (i.e., hypersensitivity). However, due to the lack of an appropriate animal model of ocular chlamydial infections, the immunological response to chlamydial antigens during infection and challenge has not been characterized. We have chosen to study the immunopathology of guinea pig inclusion conjunctivitis (GPIC) , a Chlamydia psittaci strain which produces ocular and genital infections in guinea pigs. We have characterized GPIC primary infection clinically and are in the process of characterizing the histopathology of the conjunctiva during primary infection. In addition, we have characterized a delayed type hypersensitivity response to high doses of viable GPIC and to a Triton Buy Anaprox X-100 extract of GPIC. GPIC recombinant clones producing cutaneous hyper- sensitivity in immune animals are being characterized to identify the chlamydial antigen which elicits the hypersensitivity response. The immunodominant antigens during primary infection and following challenge have been identified and recom- binant clones expressing these proteins have been isolated. 12-24 PHS Order Anaprox Online 604 Rev "H-i ZOl AI Project Number Sunmary 00061-23 00063-15 00071-14 00082-24 00201-06 00231-04 00232-04 00268-04 00402-02 LABORATORY OF PATHOBIOLOGY Rocky Mountain Laboratories Hamilton, Montana 1985 Annual Report Table of Contents Natural History of Tick-borne Rickettsiae and Their Public Health Significance — Burgdorfer Immune Responses to Rickettsial Infections — Peacock Studies on Pertussigen — Munoz Relation of Viruses to the Genesis of Chronic Disorders — Hadlow Structural Characterization of Integrated Viral Genomes — Garon
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